Problem:
Epidermodysplasia Verruciformis has no cure because it derives from a disease that doesn’t have a cure either, HPV. There is a vaccine, for HPV, however once an innocent, disturbed human feces receives it, they’re doomed! EV uprises from HPV by the inactive or mutation of cell membrane channels TMC-6 and TMC-8. We have figured out a possible solution to the backstage of HPV, the great danger of Epidermodysplasia Verruciformis.
Previous Attempted Solutions:
Solutions attempted before us included the use of surgical removal. However, since the root of the problem was not solved, the warts usually grow back. Although, some people have had the warts removed, and have not seen them grow back.
Biotech Product (Protein)
Through isolating EVER1 and EVER2’s topogenic sequence we can find the exact combination of the 25 amino acids that make it. After, we can put cells into a cell culture and instruct them to make that one protein.
Why it works
EVER1 and EVER2 are proteins involved in the transport of zinc into the cell through channels TMC-6 and TMC-8. Zinc is essential to a cell because zinc is one of the few substances that attacks foreign or defective material that made it past the cell membrane. With the immune system suppressed and zinc transports shut down, foreign materials are free to infect every single individual cell. This is also why a surgery will be needed to make sure the blood transfused blood does not become infected too.
Proposed Solution:
The the perfect end result would be a patient cured from Epidermodysplasia Verruciformis. How we will be doing this is through something called a conditional knockout. What this will do is ultimately target the infected gene in DNA and eliminate it. The way a conditional knockout works is a Cre-Lox recombination is injected into the bloodstream. It will target the infected section of DNA (gene). Next an injection of cre-recombinase, a viral enzyme that eliminates targeted infected genes, that will destroy the infected gene.
Until the patient receives a full blood replacement with blood containing fully functional TMC-6 and TMC-8 they will receive multiple injections of EVER1 and EVER2 proteins so the infection does not return. The blood replacement will not have risk of being infected because the viral enzyme in the DNA will have been removed.
Citations:
Kao, Grace F. “Epidermodysplasia Verruciformis” August 11, 2014
http://emedicine.medscape.com/article/1131981-overview
Geneoway.com “Custom Knockout Mouse Models”
https://www.genoway.com/services/customized-mouse/knockout-models/conditional-ko-tissue.htm
Ingenious Laboratory “Conditional Knockout Mouse Models”
http://www.genetargeting.com/products-and-services/types-of-mouse-models/conditional-knockout-mouse-models/
Molecular Biology 4th edition “Insertion of Membrane Proteins into the ER Membrane”
http://www.ncbi.nlm.nih.gov/books/NBK21731/
Epidermodysplasia Verruciformis has no cure because it derives from a disease that doesn’t have a cure either, HPV. There is a vaccine, for HPV, however once an innocent, disturbed human feces receives it, they’re doomed! EV uprises from HPV by the inactive or mutation of cell membrane channels TMC-6 and TMC-8. We have figured out a possible solution to the backstage of HPV, the great danger of Epidermodysplasia Verruciformis.
Previous Attempted Solutions:
Solutions attempted before us included the use of surgical removal. However, since the root of the problem was not solved, the warts usually grow back. Although, some people have had the warts removed, and have not seen them grow back.
Biotech Product (Protein)
Through isolating EVER1 and EVER2’s topogenic sequence we can find the exact combination of the 25 amino acids that make it. After, we can put cells into a cell culture and instruct them to make that one protein.
Why it works
EVER1 and EVER2 are proteins involved in the transport of zinc into the cell through channels TMC-6 and TMC-8. Zinc is essential to a cell because zinc is one of the few substances that attacks foreign or defective material that made it past the cell membrane. With the immune system suppressed and zinc transports shut down, foreign materials are free to infect every single individual cell. This is also why a surgery will be needed to make sure the blood transfused blood does not become infected too.
Proposed Solution:
The the perfect end result would be a patient cured from Epidermodysplasia Verruciformis. How we will be doing this is through something called a conditional knockout. What this will do is ultimately target the infected gene in DNA and eliminate it. The way a conditional knockout works is a Cre-Lox recombination is injected into the bloodstream. It will target the infected section of DNA (gene). Next an injection of cre-recombinase, a viral enzyme that eliminates targeted infected genes, that will destroy the infected gene.
Until the patient receives a full blood replacement with blood containing fully functional TMC-6 and TMC-8 they will receive multiple injections of EVER1 and EVER2 proteins so the infection does not return. The blood replacement will not have risk of being infected because the viral enzyme in the DNA will have been removed.
Citations:
Kao, Grace F. “Epidermodysplasia Verruciformis” August 11, 2014
http://emedicine.medscape.com/article/1131981-overview
Geneoway.com “Custom Knockout Mouse Models”
https://www.genoway.com/services/customized-mouse/knockout-models/conditional-ko-tissue.htm
Ingenious Laboratory “Conditional Knockout Mouse Models”
http://www.genetargeting.com/products-and-services/types-of-mouse-models/conditional-knockout-mouse-models/
Molecular Biology 4th edition “Insertion of Membrane Proteins into the ER Membrane”
http://www.ncbi.nlm.nih.gov/books/NBK21731/